Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein
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چکیده
منابع مشابه
Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein
The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcioning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was tr...
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Clones containing the pbpB gene which encodes penicillin-binding protein (PBP) 3 of Pseudomonas aeruginosa were detected by hybridization by PCR amplification with primers based on the conserved sequences of high-molecular-weight PBPs. The translated amino acid sequence demonstrated 45% identity and had a total of 66% conserved amino acids relative to the Escherichia coli PBP3. The pbpB gene wa...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1988
ISSN: 0378-1097
DOI: 10.1016/0378-1097(88)90367-9